FAE1 an Early Seed-Specific Promoter
This FAE I promoter technology provides seed-specific expression in plants, offering a competitive alternative to the widely used napin and phaseolin promoters.
FAE1 promoter constructs can be used in transgenic plants to promote expression of heterologous, agronomically-valuable sequences in developing seeds. Offers a competitive alternative to the widely used napin and phaseolin promoters. Can affect seed lipid metabolism, seed development, and protein or carbohydrate composition and accumulation. Can produce modified seeds with novel recombinant proteins which have pharmaceutical, industrial, or nutritional value.
Researchers at The University of British Columbia have isolated and characterized transcriptional regulatory regions derived from the Fatty Acid Elongation 1 (FAE1) genes that encode the FAE1 condensing enzymes involved in plant very long chain fatty acid (VLCFA) biosynthesis. The promoters modulate the expression of genes in developing plant embryos. These constructs may be used in transgenic cells or plants to promote the expression of foreign and endogenous genes in developing seeds thereby mediating gene expression that affects seed lipid metabolism. Additionally, the promoters can alter seed protein composition, seed carbohydrate composition, or seed development. The transcriptional regulatory region may also be useful for the production of modified seeds containing novel recombinant proteins which have pharmaceutical, industrial or nutritional value. The following image shows the position of the FAE1 gene and the FAE1 5’ promoter region pFAE1 with respect to the upstream annotated genes T4L20.80 and T4L20.90 within the BAC clone T4L20.