T Cell Receptor Zeta Chain Antibody

The invention is a novel monoclonal antibody specific for the T cell receptor zeta chain and is referred to as G3.

Advantages

  • The G3 antibody can be utilized for Immunoprecipitation of both phosphorylated and non-phosphorylated TCR zeta chain from human and mouse T cell lines.
  • The G3 antibody can be used for western blot detection of TCR zeta chain.
  • The G3 antibody can be used for the study of structural and signaling characteristics of TCR zeta chain.

Technology Details

The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma, delta, epsilon and zeta subunits, all of which are required for efficient surface expression.  T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines.  Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.

Researchers at The University of British Columbia have developed a novel monoclonal antibody, G3, which is specific for the TCR zeta chain subunit.  The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain.  The G3 monoclonal antibody will be useful for elucidating the structural and signaling characteristics of the TCR zeta chain.