Quantification of Neuraminidase (NA)

Titration method to accurately and quickly determine amount of active NA in
a sample without needing to know specific activity

Advantages

  • Enables quantification of total active NA
  • Proven to work on vaccines & single strains
  • Convenient titration method
  • Real-time results (fast < 5 min)

Technology Details

The influenza virus represents a major health burden worldwide, both in terms of annual epidemics and occasional global pandemics. Global estimates assume that about 500,000 people die due to influenza each year. The influenza virus has two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are essential to the process of infecting the host and therefore represent a major target for influenza therapeutics and vaccines.

While the sample content of HA can be determined by single-radial-immunodiffusion and the specific receptor activity by using glycan microarray-based assays, reliable and convenient techniques for measuring the concentration of active NA in a sample are currently not available. Both for studying emerging influenza strains and for understanding the contribution of NA to influenza vaccine effectiveness, it is critical to develop a general and convenient method to quantify active NA in samples, which has also been emphasized by the WHO.

Researchers at The University of British Columbia have designed a convenient titration method that enables the measurement of precise concentrations of neuraminidase in a sample for the first time. The method is quick, providing results in less than
5 minutes and does not require knowledge of the specific activity of the neuraminidase (Fig. 1).

Fig. 1: Method. Upon mixing the reagent with NA, a fluorescent signal is released, which is analyzed to give the amount of NA in the sample.